January 31, 2005
Quote of the Day
I tend toward the variation "If you can't say something nice about someone... I'm all ears."
Ward Griffiths
January 23, 2005
Quote of the Day
The first principle is that you must not fool yourself -- and you are the easiest person to fool. So you have to be very careful about that. After you've not fooled yourself, it's easy not to fool other scientists. You just have to be honest in a conventional way after that.
I would like to add something that's not essential to the science, but something I kind of believe, which is that you should not fool the layman when you're talking as a scientist. I am not trying to tell you what to do about cheating on your wife, or fooling your girlfriend, or something like that, when you're not trying to be a scientist, but just trying to be an ordinary human being. We'll leave those problems up to you and your rabbi. I'm talking about a specific, extra type of integrity that is not lying, but bending over backwards to show how you're maybe wrong, that you ought to have when acting as a scientist. And this is our responsibility as scientists, certainly to other scientists, and I think to laymen.
For example, I was a little surprised when I was talking to a friend who was going to go on the radio. He does work on cosmology and astronomy, and he wondered how he would explain what the applications of his work were. "Well", I said, "there aren't any". He said, "Yes, but then we won't get support for more research of this kind". I think that's kind of dishonest. If you're representing yourself as a scientist, then you should explain to the layman what you're doing -- and if they don't support you under those circumstances, then that's their decision.
One example of the principle is this: If you've made up your mind to test a theory, or you want to explain some idea, you should always decide to publish it whichever way it comes out. If we only publish results of a certain kind, we can make the argument look good. We must publish BOTH kinds of results.
Richard Feynman
"Cargo Cult Science"
January 22, 2005
Quote of the Day
Capitalism is the only moral social system because it allows each man to work for his own profit and because under a capitalistic system men only have to work with each other through voluntary action for mutual benefit. Capitalism maximizes wealth, prosperity and happiness.
Valara Forsythe
"Rapid Gene Synthesizer Will Enable Custom Microbe Construction"
Thanks to Perry for bringing this to my attention: "Rapid Gene Synthesizer Will Enable Custom Microbe Construction."
January 21, 2005
My first DNA electrophoresis
I am really, really enjoying my biology class, a concentrated term of cell & molecular biology. Students in this program spend about four times as much time in lab, learning industrially useful techniques, as do students in comparable programs in the University of California system. In the last three weeks, I've had hands-on time doing protein electrophoresis, conjugation (bacterial DNA transfer), and DNA electrophoresis. Here's an image of our team's first DNA gel:
The DNA is from purified coliphage Lambda virus, 48,502 Kb (kilobases) in length. Lane 1 is pure, uncut DNA. Lane 2 is DNA restricted (cut) by Eco RI enzyme, Lane 3 restricted by Hind III, and Lane 4 by both (the restriction sites are different, resulting in more, smaller DNA fragments.)
Lanes 5 through 7 are subsamples taken from 2 through 4, subjected slowly and thoroughly to the action of the enzyme DNA ligase, resulting in outrageously long, randomly recombinant strands.
The gel is purified agarose treated with ethidium bromide. The image above is a high-contrast Polaroid of the gel UV-transilluminated to fluoresce in the visible spectrum (reddish orange, here shown in black and white).
This stuff is outrageously fun.
